Coding

Part:BBa_K1352012:Design

Designed by: Martyna Sroka, Ana-Maria Cujba   Group: iGEM14_Aberdeen_Scotland   (2014-10-02)


Ag43+FLAG+MCS(-)beta-hairpins


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 745
    Illegal NgoMIV site found at 1867
    Illegal NgoMIV site found at 2377
    Illegal NgoMIV site found at 2398
    Illegal AgeI site found at 1393
    Illegal AgeI site found at 2137
    Illegal AgeI site found at 2551
    Illegal AgeI site found at 2793
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Maintenance of the in-frame ORF


Source

PCR amplified from BioBrick Bba_K1352001; beta-hairpins were removed via In-Fusion (Clonetech).

References